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[Distribution of minimal residual leukemia in Brown Norway rats express LacZ gene]
1Department of Hematology, Huashan Hospital, Shanghai Medical University, Shanghai 200040, China.
Objective:
To investigate the distribution of minimal residual leukemia (MRL) in vivo.
Methods:
The MRL model in BN rat was established by using LT12nl leukemia cell line carrying LacZ gene. The marker gene (LacZ) was detected by using reverse transcriptase polymerase chain reaction (RT-PCR) and nested PCR, X-gal staining, and cytomorphological and pathological examination.
Results:
PCR assays showed positive result in humerus at day 4, femur at day 6, and spleen at day 9 after CTX treatment. The peak positive signal in humerus was stronger at day 9. X-gal staining showed negative in spleen at day 9. PCR showed negative results in peripheral blood and liver.
Conclusion:
PCR can be used as an effective tool to investigate the distribution pattern in experimental MRL.
