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[Antigen loading on dendritic cells affects the cell function in stimulating T cells]
Objective:
To study the effect of antigen loading on dendritic cells (DC).
Methods:
DCs collected from peripheral blood monocytes were loaded with a tumor antigen from XG-7 cell line. These DCs were then co-cultured with allogeneic T cells and were compared with those DCs without antigen exposure.
Results:
Although DCs showed no change in their phenotypes after cultured with the antigen, they secreted more IL-12, and became more powerful in allogenic mixed lymphocyte reaction (MLR). Antigen-loaded DC stimulated more CD(4)(+) cells to proliferate than nonantigen-loaded DCs did. These CD(4)(+) cells did not kill XG-7 cells, but promoted CD(8)(+) cells' ability to inhibit the XG-7 proliferation.
Conclusion:
The proliferation of CD(4)(+) cells after cultured with DC may become an indicator for the function of antigen loaded DC and for the efficiency of DC immunotherapy.