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Updated: Aug 14, 2026
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
Published on: May 7, 2012
[Experimental study on ex vivo expansion of CD(34)(+) umbilical cord blood cells]
1tute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
Objective:
To investigate the potential possibility that to expanse ex vivo umbilical cord blood (UCB) cells to an amount sufficient adults transplantation.
Method:
Purified CD(34)(+) UCB cells from 10 fresh UCB samples were cultured for 7 days in IMDM mediums containing 20% FBS and one of the following three combinations: Group A (IL-1beta + IL-3 + IL-6 + G-CSF + Epo + FL), Group B (SCF + IL-1beta + IL-3 + IL-6 + G-CSF + Epo) and Group C (FL + SCF + IL-1beta + IL-3 + IL-6 + G-CSF + Epo).
Result:
(1) There was no significant difference between Groups A and B in expansion of UCB cells. But in group C, the expansion was greater than that in group A (P < 0.01) or group B (P < 0.05). The effects of FL and SCF were synergistic. (2) Over 50 x 10(6) CD(34)(+) cells which are sufficient for adult transplantation were obtained in 30% of UCB samples in group C. (3) The expanded CD(34)(+) UCB cells retained original clonogenic efficiency, the primitive CD(34)(+)CD(38)(-) proportion and the expansion potential were the same as fresh UCB cells.
Conclusion:
The ex vivo expansion of CD(34)(+) UCB cells might provide sufficient hematopoietic stem cells for adult transplantation.
