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[Long-term expression of MHC-I gene in recipient mice]
1Laboratory of Surgery, The First Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510080, China.
Objective:
To explore the possibility of long-term expression of transferred MHC-I gene in recipient mice.
Methods:
MHC-I gene of the donor mouse (C(57)BL/6) was transferred into the recipient (BALB/c) hematopoietic cells by retrovirus-mediated gene transfer technique, and the gene transferred cells were reinfused into the recipient mice. PCR, RT-PCR and FACS were used to assay the long-term expression of MHC-I gene in the recipient mice.
Results:
The gene transferred mice hematopoietic cells and their G418-resistant CFU-GM were demonstrated to be able to express the MHC-I gene. The cells proliferation and differentiation had no change. The MHC-I gene could be expressed for a longer period in the recipient mice.
Conclusion:
This experiment makes ready for the further research on inducing transplantation tolerance through MHC-I gene transfer.