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Updated: Oct 2, 2026
07:42
Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
[Chromosome t (8; 21) and t (15; 17) in a patient with acute myeloid leukemia]
Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi
|March 6, 2002
Abstract
Objective:
To report an acute myeloid leukemia (AML) patient with both chromosome t (8; 21) and t (15; 17).
Methods:
Chromosome specimen was prepared by short-term culture of bone marrow cells, karyotype analyses by R-banding technique, and fusion genes by reverse transcriptase-polymerase chain reaction (RT-PCR).
Result:
Karyotype analyses showed the appearance of 45, X, -X, t (8; 21), t (15; 17), and RT-PCR assay revealed AML1/ETO and PML-RARalpha fusion gene transcripts.
Conclusion:
AML with both t (8; 21) and t (15; 17) might be recognized as a a novel subtype of acute leukemias.
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