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[Affect of As(2)O(3) on the sister chromatid exchanges in human blood lymphocytes]
1Department of Hospital Administration, Yunnan Armed Police Hospital, Kunming 650111, China.
Objective:
To assess the potential of As(2)O(3) in inducing malignant transformation at its therapeutic concentrations.
Methods:
The sister chromatid exchange (SCE) in human peripheral blood lymphocytes (PBL) treated with different concentrations of As(2)O(3) was detected by the staining technique for sister chromatid differentiation.
Results:
At 2 x 10(-6)mol/L and 1 x 10(-6) mol/L of As(2)O(3), the SCE frequency in PBLs was significantly increased as compared with the control (P < 0.01). Most of the lymphocytes were arrested at the first cell cycle (M1) and the mitotic index was significantly decreased in a dose dependent manner. At 1 x 10(-7) mol/L or less of As(2)O(3), the SCE frequency, the cell proliferation kinetics and the mitotic index in PBLs were similar to the control (P > 0.05).
Conclusion:
It seemed that As(2)O(3) might induce transformation and affect the function of lymphocytes when used within the therapeutical concentration range.