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Proton diffusion at phospholipid assemblies.

Jie Zhang1, Patrick R Unwin

  • 1Contribution from the Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.

Journal of the American Chemical Society
|March 7, 2002
PubMed
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A new scanning electrochemical microscopy proton feedback method reveals significant lateral proton diffusion in acidic phospholipid monolayers (DPPS). Zwitterionic (DPPC) monolayers show no detectable proton diffusion, highlighting the role of acid-base properties.

Area of Science:

  • Electrochemistry
  • Surface Science
  • Biophysics

Background:

  • Phospholipid monolayers at interfaces are crucial in biological systems.
  • Understanding proton dynamics at these interfaces is key to cellular processes.
  • Current methods for studying interfacial proton diffusion are limited.

Purpose of the Study:

  • To develop a novel scanning electrochemical microscopy (SECM) proton feedback method.
  • To investigate lateral proton diffusion in phospholipid monolayers.
  • To compare proton diffusion in acidic (DPPS) versus zwitterionic (DPPC) monolayers.

Main Methods:

  • Utilized a "submarine" ultramicroelectrode (UME) in a Langmuir trough.
  • Electrogenerated a base to perturb and detect proton concentrations.

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  • Developed a numerical model accounting for interfacial acid-base properties.

    • Main Results:

    • Demonstrated significant lateral proton fluxes in dipalmitoyl-L-alpha-phosphatidyl-L-serine (DPPS) monolayers.
    • Observed a lower lateral proton diffusion coefficient in DPPS compared to bulk solution.
    • Found no detectable lateral proton diffusion in dipalmitoyl-L-alpha-phosphatidylcholine (DPPC) monolayers.

    Conclusions:

    • The developed SECM proton feedback method is effective for studying interfacial proton diffusion.
    • Acid-base properties of phospholipids significantly influence interfacial proton transport.
    • Lateral proton diffusion is substantial in acidic phospholipids but negligible in zwitterionic ones.