New imaging modes for lenslet-array tandem scanning microscopes
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A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
T F Watson1, R Juskaitis, T Wilson
1Division of Conservative Dental Surgery, Guy's, King's & St Thomas' Dental Institute, Guy's Hospital, London, SE1 9RT, UK.
Abstract:
The tandem scanning microscope permits confocal images to be obtained in real time and viewed directly by eye. The light budget of these instruments may be increased from a few percent to a few tens of percent by incorporating an array of microlenses so as to increase the amount of illumination light that reaches the specimen. These instruments are configured for fluorescence imaging together with laser illumination. We describe how the versatility of the instrument may be enhanced to permit the use of incoherent light sources as well as extending the imaging modes to include bright-field reflection.
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