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Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
Published on: November 20, 2018
Identification of a polymorphic microsatellite marker in the mouse Fas ligand gene
M Lévi-Strauss1, C Beauvais, S Karray
1Institut National de la Sante et de la Recherche Médicale (INSERM), Unité 25, Hĵpital Necker Enfants Malades, Paris, France.
Abstract:
We describe the characterization of a polymorphic microsatellite marker, located 1.8 kb downstream of exon 4 in the mouse Fas ligand gene. This (GT) repeat sequence allows the identification of four alleles which can very easily be distinguished by simple agarose electrophoresis.
