Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

G protein specificity: traffic direction required.

Paul R Albert1, Liliane Robillard

  • 1Ottawa Health Research Institute, Neuroscience, University of Ottawa, 451 Smyth Road, K1H-8M5, Ottawa, ON, Canada. palbert@uottawa.ca

Cellular Signalling
|March 8, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Greening the lab: fighting climate change to enhance mental health.

Journal of psychiatry & neuroscience : JPN·2025
Same author

Rapid reorganization of serotonin projections and antidepressant response to 5-HT1A-biased agonist NLX-101 in fluoxetine-resistant cF1ko mice.

Neuropharmacology·2024
Same author

CaMKIV-Mediated Phosphorylation Inactivates Freud-1/CC2D1A Repression for Calcium-Dependent 5-HT1A Receptor Gene Induction.

International journal of molecular sciences·2024
Same author

Chronic Desipramine Reverses Deficits in Cell Activity, Norepinephrine Innervation, and Anxiety-Depression Phenotypes in Fluoxetine-Resistant cF1ko Mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2023
Same author

Transcriptional Regulation of the Human 5-HT1A Receptor Gene by Lithium: Role of Deaf1 and GSK3β.

International journal of molecular sciences·2023
Same author

PFTK1 kinase regulates axogenesis during development via RhoA activation.

BMC biology·2023
Same journal

Integrative spatiotemporal analysis uncovers an Fto-mediated epigenetic-metabolic axis governing myocardial ischemic injury.

Cellular signalling·2026
Same journal

Caspase-3 activation is a brake in GSDMD-mediated pyroptosis.

Cellular signalling·2026
Same journal

ILF3 promotes proliferation and invasion of hepatocellular carcinoma cells through STAT3 phosphorylation and formation of an endogenous complex with SRPK1.

Cellular signalling·2026
Same journal

Extracellular regucalcin blocks the proliferation and metastatic activity of SK-N-SH human neuroblastoma cells by targeting diverse signaling pathways: Involvement in the cancer cell microenvironment.

Cellular signalling·2026
Same journal

Neutrophilic LGALS2 drives tuberculosis susceptibility by regulating Th2 polarization via the IFN-γ-HLA-II-CD4 axis.

Cellular signalling·2026
Same journal

Diosgenin alleviates radiation nephropathy by suppressing renal mTORC1 signalling with concomitant effects on the gut and liver.

Cellular signalling·2026
See all related articles

Specific G protein subunits (Galpha and Gbetagamma) are crucial for signaling pathways. Understanding their precise coupling specificity can lead to targeted drug development for various receptors.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Pharmacology

Background:

  • Pertussis toxin (PTX)-sensitive G(i/o) proteins regulate critical signaling pathways, including ion channel activity.
  • These G proteins are composed of Galpha and Gbetagamma subunits, which interact with various receptors and effectors.
  • The precise mechanisms governing the specificity of these interactions are complex and not fully elucidated.

Purpose of the Study:

  • To review the coupling specificity of Galpha and Gbetagamma subunits within PTX-sensitive G(i/o) proteins.
  • To explore the implications of this specificity for receptor-effector networks and signaling pathways.
  • To identify potential therapeutic targets based on understanding G protein specificity.

Main Methods:

  • Literature review of studies investigating G protein subunit interactions.

Related Experiment Videos

  • Analysis of evidence from both in vitro and intact cellular systems.
  • Examination of structural and scaffolding factors influencing receptor-G protein specificity.

    • Main Results:

    • Evidence suggests specific combinations of Galpha and Gbetagamma subunits are essential for distinct receptor-mediated signaling.
    • Higher specificity for Galpha and Gbetagamma interactions is observed in intact biological systems compared to in vitro assays.
    • The structural basis for receptor-G protein specificity is not completely understood, involving interaction domains and scaffolding processes.

    Conclusions:

    • Understanding G protein subunit coupling specificity is key to deciphering complex signaling networks.
    • This specificity is more pronounced in vivo, highlighting the importance of cellular context.
    • Identifying pathway-specific G protein interactions could enable the development of novel, targeted therapeutic ligands.