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Related Experiment Videos

Function-specific calcium stores selectively regulate growth hormone secretion, storage, and mRNA level.

James D Johnson1, Christian Klausen, Hamid R Habibi

  • 1Department of Biological Sciences, University of Alberta, Edmonton, T6G 2E9, Canada T2N 1N4.

American Journal of Physiology. Endocrinology and Metabolism
|March 8, 2002
PubMed
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Intracellular calcium (Ca(2+)) stores independently regulate goldfish growth hormone (GH) release and production. Different Ca(2+) stores control distinct aspects of the secretory pathway, impacting GH secretion and content.

Area of Science:

  • Endocrinology
  • Cell Biology
  • Molecular Biology

Background:

  • Intracellular calcium (Ca(2+)) signaling is crucial for regulating cellular processes, including hormone release.
  • Specific Ca(2+)-sensitive pathways within the secretory pathway remain incompletely understood.

Purpose of the Study:

  • To investigate the distinct roles of independent intracellular Ca(2+) stores in regulating growth hormone (GH) release, storage, and mRNA levels in goldfish somatotropes.
  • To determine if Ca(2+) signals are directly coupled to GH exocytosis or influence long-term secretory function.

Main Methods:

  • Utilized thapsigargin to deplete endoplasmic reticulum Ca(2+) stores and activate the sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase.
  • Employed ryanodine (Ry) to modulate ryanodine receptors (RyRs), another type of intracellular Ca(2+) store.

Related Experiment Videos

  • Assessed GH release, GH content, and GH mRNA levels following various Ca(2+) store manipulations.

    • Main Results:

    • Thapsigargin-induced Ca(2+) signals did not acutely increase GH release, indicating a dissociation between Ca(2+) influx and exocytosis.
    • Long-term thapsigargin treatment led to steady GH secretion but decreased GH content, without altering GH mRNA.
    • Ryanodine receptor activation/inhibition differentially affected GH release, content, and mRNA, suggesting distinct roles for RyRs.
    • Elevating intracellular Ca(2+) independently of stores via KCl reduced GH mRNA levels.

    Conclusions:

    • Biochemically distinct intracellular Ca(2+) stores independently regulate specific components of the secretory pathway in goldfish somatotropes.
    • The findings suggest a complex interplay between different Ca(2+) signaling pathways and the regulation of GH synthesis and secretion.