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The Enterococcus faecalis gene encoding the novel general stress protein Gsp62
Alain Rincé1, Marilyne Uguen2, Yoann Le Breton1
1Laboratoire de Microbiologie de l'Environnement, IRBA, Université de Caen, 14032 Caen Cedex, France1.
Microbiology (Reading, England)
|March 8, 2002
Summary
Enterococcus faecalis general stress protein Gsp62 is induced by various environmental stresses. However, disrupting the gsp62 gene did not significantly impact cell survival, suggesting complex stress response mechanisms.
Area of Science:
- Microbiology
- Molecular Biology
- Stress Response
Background:
- Enterococcus faecalis possesses a general stress protein, Gsp62.
- Understanding the regulation and function of stress proteins is crucial for bacterial survival.
Purpose of the Study:
- To characterize the general stress protein Gsp62 in Enterococcus faecalis.
- To investigate the transcriptional regulation and functional role of gsp62 under various stress conditions.
Main Methods:
- Purification of Gsp62 using two-dimensional gel electrophoresis.
- N-terminal amino acid sequencing and gene analysis.
- Transcriptional analysis (mRNA, 5' RACE PCR, lacZ fusions).
- Gene disruption and stress survival assays.
Main Results:
- Gsp62 is a 172 amino acid protein encoded by a monocistronic mRNA.
- gsp62 expression is induced by stationary phase, heat shock, acid pH, detergents, ethanol, and peroxides.
- gsp62 disruption did not affect survival under individual stress conditions.
- An upstream inverted repeat is necessary for induction by environmental stresses, but not stationary phase entry.
Conclusions:
- Gsp62 is a stress-induced protein in Enterococcus faecalis.
- Two distinct regulatory mechanisms control gsp62 induction: one for environmental stresses and another for stationary phase.
- The functional significance of Gsp62 under individual stress conditions requires further investigation.