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[Histone H3 gene expression level as markers of hyperplasia simplex of endometrium]
1Katedry i Zakładu Biologii Molekularnej, Biochemii i Biofarmacji SLAM w Sosnowcu.
Subject:
The excessive proliferative activity of endometrial cells drives to hyperplasia, via states: simplex hyperplasia, complex and atypical hyperplasia, and finally preneoplastic state may arise. New molecular markers and their ability to differentiate physiological and pathological states are described.
Design:
The aim of this study was to evaluate the usefulness of histone H3 gene expression level as a marker of hyperplasia of endometrium.
Material And Methods:
The level of H3 mRNA was estimated using RT-QPCR method in proliferative phase (n = 5) and endometrial hyperplasia simplex (n = 8). Differences in expression level of H3, were evaluated by Student's t-test (t-test for independent samples).
Results:
In our experiments the average mRNA H3 expression was significantly higher(p < 0.05, p = 0.03) in case of hyperplasia simplex in comparison to H3 expression in proliferative state of endometrium.
Conclusion:
The results confirm advantage of estimation of H3 mRNA level as a marker of cell proliferation in endometrium.