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Characterization of toxin from cheilanthes fern and its effect on lymphocyte proliferation and DNA fragmentation
K A Kumar1, M Kataria, R Somvanshi
1Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar.
Abstract:
Thin layer chromatography of aqueous extract of whole Cheilanthesfarinosa fern indicated the presence of ptaquiloside or ptaquiloside like compound, coinciding Rf values with that of Pterosin B standard. HPLC analysis revealed the presence of 26.3 mg/kg ptaquiloside. In vitro studies of the aqueous extract on lymphocyte culture revealed a correlation between stimulative indices and concentration of aqueous extract. Stimulation in lymphocyte proliferation was in order of bracken > cheilanthes > ConA> ptaquiloside standard. On incubation of lymphocyte with aqueous extract of ferns, no DNA damage was observed in isolated DNA.
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