Partial characterization of a cerebral thyroid hormone-responsive protein

Michael J Haas¹, Jian-Ping Li, Kristoffer Pun

¹Division of Endocrinology, Diabetes and Metabolism, St. Louis University School of Medicine, St. Louis, Missouri 63104, USA.

|March 9, 2002

Summary

Thyroid hormone-responsive protein (THRP) and Abi-2 share high sequence similarity but exhibit distinct tissue distribution and biological properties. THRP is tyrosine phosphorylated but not a substrate for c-Abl tyrosine kinase.

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Thyroid hormone-responsive protein (THRP) shares high sequence homology with c-Abl interactor protein 2 (Abi-2).
Abi-2 is known to be a substrate for c-Abl tyrosine kinase activity.
Understanding the relationship between THRP and Abi-2 is crucial for elucidating their distinct biological roles.

Purpose of the Study:

To determine if THRP is a rat homologue of Abi-2 or a distinct protein.
To investigate the tissue distribution of THRP and Abi-2 mRNA.
To characterize the phosphorylation status and c-Abl interaction of THRP.

Main Methods:

Ribonuclease protection assay to quantify THRP and Abi-2 mRNA levels in rat tissues (cerebral, hepatic, heart, small intestine).
Transfection of PC12 cells with THRP cDNA to assess tyrosine phosphorylation.
Co-transfection experiments with THRP and c-Abl (wild-type and mutant) in PC12 cells.

Main Results:

THRP mRNA expression is significantly higher in cerebral tissue compared to Abi-2 mRNA.
Hepatic expression shows a reversed ratio, with much higher Abi-2 mRNA than THRP mRNA.
THRP is tyrosine phosphorylated in PC12 cells but is not a substrate for c-Abl kinase activity, unlike Abi-2.

Conclusions:

THRP and Abi-2 possess distinct tissue-specific expression patterns.
THRP and Abi-2 exhibit unique biological properties, particularly regarding their interaction with c-Abl.
THRP is a distinct protein from Abi-2, with its own functional characteristics.

Related Experiment Videos

Partial characterization of a cerebral thyroid hormone-responsive protein

Related Concept Videos

Sex-specific association of plasma vitamin E with homocysteine concentrations in Chinese middle-aged and older men and women: evidence from pooled population-based study.

Identification of the Novel HLA-A*30:112 Allele by Sequencing-Based Typing in a Chinese Individual.

Reframing the High-density Lipoprotein (HDL) Hypothesis in Light of Clinical Trials of Cholesterol Ester Transfer Protein (CETP) Inhibitors.

An endothelial-centered regulatory framework reveals context-dependent roles of MYLK in lung adenocarcinoma.

Integrating Bioinformatics and Experimental Validation Identify CCNA2 as a Novel Prognostic Biomarker and Tumor Promoter via the PI3K/AKT Pathway in Lung Adenocarcinoma.

The Novel HLA-B*35:293 Allele, Identified by Sanger Dideoxy Nucleotide Sequencing.

GPR97-Gα12-mTORC1 signaling drives myotube hypertrophy: identification of 5-hydroxy-7-methoxyflavone as a pathway activator.

Citrullination in proteins of the polycomb repressor complex 1.

A novel dibenzoylmethane derivative (IDPIP) impairs melanoma cell migration and selectively induces apoptosis in vitro.

Myco-assisted Zinc Oxide Nanoparticles by Aspergillus sp. SA18: Chemical profiling, antimicrobial, anticancer, and Computational Insights into GyrA and GyrB Inhibition with ADME physicochemical properties.

CLOCK confers EGFR-TKIs resistance in non-small cell lung cancer by transcriptionally upregulating LAMC2 expression and activating multiple kinase signaling.

Protective effects of 17β-Estradiol (E2) against macrophage ferroptosis via tubular epithelial cells derived exosomal HDAC2 mediated H3K27 deacetylation at the GPX4 promoter during sepsis-induced renal injury.

GRIN1-related epilepsy in a neonate with response to memantine and vigabatrin.

Injectable Supramolecular Hydrogel Encapsulating CRISPR-Engineered MSCs Drives Synergistic Neuroprotection and Functional Recovery After Traumatic Brain Injury.

Intraoperative motor evoked potential changes associated with surgical trajectory adjustment during endoscopic evacuation of basal ganglia hemorrhage: A technical note with illustrative case.

Transcatheter versus surgical aortic valve replacement for severe aortic stenosis in low-risk adults: a network meta-analysis.

Global Perspectives on Endovascular Treatment of Intracranial Atherosclerotic Disease.

Integrating Near-Field Direct-Writing 3D Printing and Layer-By-Layer Self-Assembly to Construct Electroactive Anisotropic Scaffolds for Neural Tissue Engineering.

Related Experiment Videos

Partial characterization of a cerebral thyroid hormone-responsive protein

Area of Science:

Background:

Purpose of the Study:

Main Methods:

Main Results:

Conclusions:

Related Concept Videos

Related Articles