Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Boron-containing aptamers to ATP.

Susan M Lato1, Nicole D S Ozerova, Kaizhang He

  • 1Department of Chemistry, Indiana University, 800 East Kirkwood, Bloomington, IN 47405-7102, USA.

Nucleic Acids Research
|March 9, 2002
PubMed
Summary

Boron neutron capture therapy requires specific delivery agents. Researchers found that boronated nucleotides are compatible with aptamer selection processes, enabling the development of boronated aptamers for cancer treatment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DUSP11 is an Intracellular Innate Immune Checkpoint in Lung Adenocarcinoma.

Cancer immunology research·2025
Same author

Anti-EGFR aptamer exhibits direct anti-cancer effects in NSCLC cells harboring EGFR L858R mutations.

NPJ precision oncology·2024
Same author

A Branched SELEX Approach Identifies RNA Aptamers That Bind Distinct HIV-1 Capsid Structural Components.

ACS infectious diseases·2024
Same author

Multiplexed <i>In Vivo</i> Screening Using Barcoded Aptamer Technology to Identify Oligonucleotide-Based Targeting Reagents.

Nucleic acid therapeutics·2024
Same author

Differentiation SELEX approach identifies RNA aptamers with different specificities for HIV-1 capsid assembly forms.

bioRxiv : the preprint server for biology·2024
Same author

Post-transcriptional capping generates coenzyme A-linked RNA.

RNA biology·2023

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Boron neutron capture therapy (BNCT) is an experimental cancer treatment.
  • Highly specific delivery agents are needed for BNCT.
  • Nucleic acid aptamers are known for their specific molecular targeting capabilities.

Purpose of the Study:

  • To investigate the impact of boronated nucleotide analogs on RNA function.
  • To assess the compatibility of boronated nucleotides within the SELEX process.
  • To explore the potential of creating boronated aptamers for BNCT.

Main Methods:

  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX) was employed.
  • Boronated nucleotide analogs, guanosine 5'-(alpha-P-borano) triphosphate (bG) and uridine 5'-(alpha-P-borano) triphosphate (bU), were substituted for their natural counterparts (GTP and UTP).

Related Experiment Videos

  • Aptamer selections were performed using C8-linked ATP agarose as the target.

    • Main Results:

    • Substitution of bG or bU diminished or eliminated target recognition in some known aptamers.
    • ATP-binding aptamers with a zeta-fold structure were inactivated by bG substitution but only moderately affected by bU.
    • SELEX selections with bU yielded some zeta-fold aptamers, while bG selections did not.
    • Non-zeta aptamers from both bU and bG populations tolerated and often required the borano substitution.
    • The requirement for bU or bG was specific, with no cross-compatibility.

    Conclusions:

    • Boronated nucleotides (bG and bU) are fully compatible with the SELEX process.
    • These analogs can be used to generate boronated aptamers.
    • Boronated aptamers hold promise as therapeutic agents for BNCT.