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Related Experiment Videos

Tissue-engineered human auricular cartilage demonstrates euploidy by flow cytometry.

S H Kamil1, B S Aminuddin, L J Bonassar

  • 1Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, and the Department of Otology and Laryngology, Harvard Medical School, Boston, Massachusetts, USA.

Tissue Engineering
|March 12, 2002
PubMed
Summary

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Transforming growth factor-beta (TGF-beta) and basic fibroblast growth factor (bFGF) did not induce aneuploidy in cultured human chondrocytes. Tissue-engineered cartilage grown with bFGF showed the best resemblance to native cartilage.

Area of Science:

  • Tissue engineering
  • Cell biology
  • Biomaterials science

Background:

  • Growth factors like TGF-beta and bFGF stimulate chondrocyte proliferation.
  • Malignant transformation and aneuploidy are theoretical risks in growth factor-stimulated chondrocytes.
  • Aneuploidy is observed in human cartilaginous tumors.

Purpose of the Study:

  • To investigate the potential for aneuploidy in human chondrocytes cultured with TGF-beta and bFGF.
  • To evaluate the histological quality of tissue-engineered cartilage formed under these conditions.

Main Methods:

  • Human auricular chondrocytes were cultured in vitro for 6 weeks with or without TGF-beta and bFGF.
  • DNA analysis for aneuploidy was performed using flow cytometry at multiple time points.

Related Experiment Videos

  • Engineered cartilage constructs were implanted in athymic mice and examined histologically after 8 weeks.

    • Main Results:

    • Flow cytometry revealed no evidence of aneuploidy; chondrocytes maintained a diploid state.
    • Cells cultured with TGF-beta showed a persistent increase in S-phase.
    • Tissue-engineered cartilage cultured with bFGF most closely resembled native cartilage histologically.
    • TGF-beta resulted in suboptimal cartilage morphology.

    Conclusions:

    • TGF-beta and bFGF stimulation of human chondrocytes in vitro does not lead to aneuploidy.
    • bFGF promotes the formation of tissue-engineered cartilage with morphology similar to native cartilage.
    • Further analysis is recommended to fully assess chondrocyte behavior and safety.