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Related Experiment Videos

Identification of novel E2F1-regulated genes by microarray.

Yihong Ma1, Rhonda Croxton, Ronnie L Moorer

  • 1Program in Molecular Oncology, H. Lee Moffitt Comprehensive Cancer Center and Research Institute, University of South Florida, Tampa, 33612, USA.

Archives of Biochemistry and Biophysics
|March 13, 2002
PubMed
Summary

The E2F1 transcription factor activates genes for cell cycle progression. This study reveals E2F1 also impacts apoptosis, signal transduction, and surprisingly, represses other genes.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genomics

Background:

  • The E2F pathway is hypothesized to control the transition from quiescence to DNA synthesis.
  • Previous research has not rigorously tested this hypothesis on a genomic scale.

Purpose of the Study:

  • To investigate the genomic-scale effects of E2F1 on gene expression in quiescent cells.
  • To identify novel cellular functions regulated by E2F1.

Main Methods:

  • Infection of quiescent mouse fibroblasts lacking E2F1 with an adenovirus expressing E2F1.
  • Analysis of gene expression for over 6000 genes using high-density microarrays.

Main Results:

  • Microarray data support E2F1's role in regulating genes for DNA synthesis.

Related Experiment Videos

  • E2F1 was found to influence pathways involved in apoptosis, signal transduction, transcriptional control, and membrane biology.
  • A significant number of genes were identified as being repressed by E2F1, indicating a potential transcriptional repression role.

    • Conclusions:

    • E2F1 plays a key role in activating genes essential for cell cycle progression.
    • E2F1 regulates a broader range of cellular functions than previously understood.
    • E2F1 may possess a novel mechanism for repressing gene transcription.