Micro-determination of bromine in biological samples using proton-induced x-ray emission spectroscopy

The plasma level of bromine was measured by a proton-induced X-ray emission spectroscopy. Since bromine is the most difficult halogen to be determined and appears only in trace quantities, powerful techniques are required. The excellent reproducibility was obtained on 19 measurements on a sample of a single individual; the coefficient of variation was 6.3%. The average and standard deviation of plasma bromine levels on 47 young women were 0.50 and 0.10 mg/100 ml, respectively. Results by other methods in the literature were briefly reviewed.