Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane protein microarrays.

Ye Fang1, Anthony G Frutos, Joydeep Lahiri

  • 1Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA.

Journal of the American Chemical Society
|March 14, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal opioid-free anesthesia containing esketamine versus opioid-based anesthesia: a systematic review and meta-analysis.

BMC anesthesiology·2026
Same author

Efficacy, tolerability, and safety of 1.5L-lactulose as bowel preparation for patients with inflammatory bowel disease: a randomized, single-blinded trial.

Internal and emergency medicine·2026
Same author

Ellagic acid alleviates ulcerative colitis in a GPR35-dependent manner associated with SDH restoration.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

N-Glycosylation and Alzheimer's disease: A 2001-2025 global bibliometric landscape revealing emerging diagnostic trends.

Journal of Alzheimer's disease reports·2026
Same author

Association of the Albumin-Bilirubin score with 7-day incident delirium risk following bloodstream infection in critically ill adults: evidence from a propensity-weighted cohort.

BMC infectious diseases·2026
Same author

Renal Tubule-Specific Deletion of Nephrocystin 3 <i>(Nphp3)</i> Causes Infantile Nephronophthisis-like Phenotypes in Mice.

International journal of molecular sciences·2026
Same journal

Proton-Gated Torsional Spring for Molecular Energy Storage.

Journal of the American Chemical Society·2026
Same journal

Topologically Programmed Dual-Channel Covalent Organic Frameworks Decouple Gas and Ion Fluxes for Acidic CO<sub>2</sub> Electroreduction.

Journal of the American Chemical Society·2026
Same journal

Plasmonic Re-Excitation Enables Superoxide-Mediated Ethane Conversion to Acetic Acid under Visible Light.

Journal of the American Chemical Society·2026
Same journal

Photocatalytic Controlled Halodefluorination of Perfluoroalkyl Compounds Using <i>N</i>-Arylphenothiazines.

Journal of the American Chemical Society·2026
Same journal

Photoinduced Disproportionation Enables Oxidative Addition of Aryl Iodides at a Gallium(I) Center.

Journal of the American Chemical Society·2026
Same journal

Biocatalytic C3 β-<i>O</i>-Glycosylation of Triterpenes and Sterols to Synthesize Natural and Unnatural Saponins.

Journal of the American Chemical Society·2026
See all related articles

This study developed a novel method for creating G protein-coupled receptor (GPCR) microarrays for drug discovery. These arrays enable specific ligand binding and affinity studies, advancing protein microchip technology.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • G protein-coupled receptors (GPCRs) are crucial drug targets, but their study in microarrays is challenging.
  • Existing protein microchip technologies lack practical methods for membrane proteins like GPCRs.

Purpose of the Study:

  • To develop and validate a novel fabrication method for GPCR microarrays.
  • To demonstrate the utility of these arrays for specific ligand binding and selectivity studies.
  • To assess the feasibility of using GPCR arrays for determining binding affinities.

Main Methods:

  • Fabrication of microarrays by printing membrane preparations containing GPCRs onto gamma-aminopropylsilane (GAPS)-coated surfaces.
  • Demonstration of specific ligand binding using fluorescently labeled neurotensin (BT-NT) and adrenergic receptor antagonists (CGP 12177).

Related Experiment Videos

  • Assessment of receptor subtype selectivity and competitive binding assays to estimate binding affinities (IC50).

    • Main Results:

    • Successfully created stable and fluidic microarrays of model membranes and GPCRs.
    • Demonstrated specific binding of BT-NT to neurotensin receptors (NTR1) and inhibition by unlabeled neurotensin.
    • Showcased selectivity studies for adrenergic receptor subtypes (beta1, beta2, alpha2A) and accurate estimation of neurotensin binding affinity (IC50 = 2 nM).

    Conclusions:

    • The developed GPCR microarray fabrication method is effective for presenting functional membrane proteins.
    • These arrays facilitate specific ligand binding, selectivity, and binding affinity studies, filling a gap in protein microchip technology.
    • This technology holds promise for high-throughput screening and drug discovery efforts targeting GPCRs.