Isolation of Guinea pig inner hair cells using manual microsurgical dissection

Cochlear inner hair cells (IHCs) and outer hair cells (OHCs) show distinctive morphological features that are usually sufficient to distinguish these two species in vitro. However, OHCs may sometimes resemble IHCs when they are mechanically distorted or begin swelling at their basal end. As a result, accurately discriminating the cells based on morphology was thought to be problematic. An objective method that allows us to clearly and unambiguously distinguish these two cell types is therefore of continued interest. We describe a protocol in which solitary IHCs were harvested from guinea pig cochlea using a manual microsurgical dissection.