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Updated: Aug 9, 2026
Acute Myocardial Infarction in Rats
Published on: February 17, 2011
14 C-deoxyglucose imaging overestimates myocardial viability in subacute infarction of rats
S Hasegawa1, H Kusuoka, K Fukuchi
1Division of Tracer Kinetics, Department of Diagnositic Medicine, Osaka University Graduate School of Medicine, Suita, Japan. hasegawa@tracer.med.osaka-u.ac.jp
Abstract:
Clinical studies using 18F-fluorodeoxyglucose suggest that this tracer may overestimate myocardial viability. This study aimed to elucidate whether 2-deoxyglucose accurately indicates myocardial viability at the early phase of myocardial infarction. Autoradiography with 14C-deoxyglucose was performed in fasting rats whose left coronary artery was occluded for 60 min and then reperfused. 14C-deoxyglucose was injected 30 min after the reperfusion (acute; n=10) or 1 week later (subacute; n=9). Infarction and risk areas were identified by triphenyl tetrazolium chloride or haematoxylin-eosin staining and methylene blue, respectively. Immuno-histochemical staining using anti-glucose transporter 1 and 4 antibodies was performed. At the acute stage, the uptake of deoxyglucose was consistent with the grade of anti-glucose transporter 4 expression. At the subacute stage, the uptake of deoxyglucose in poorly viable myocardium (543.4+/-343.7%: normalized with the uptake at the right ventricle) as well as in the viable one (335.2+/-149.8%) in the risk area was significantly greater than that in the remote area (116.4+/-94.9%, P<0.01). Anti-glucose transporter 1 was expressed in the poorly viable area where inflammatory cells infiltrated. It is concluded that deoxyglucose uptake by inflammatory cells which express anti-glucose transporter 1 causes overestimation of myocardial viability at subacute stage.
Insights
2-deoxyglucose uptake may overestimate myocardial viability in the subacute phase of infarction. Inflammatory cells expressing glucose transporter 1 contribute to this overestimation, impacting assessments of heart muscle viability.
Area of Science:
- Cardiology
- Nuclear Medicine
- Pathology
Background:
- 18F-fluorodeoxyglucose (FDG) may overestimate myocardial viability in clinical studies.
- Accurate assessment of heart muscle viability is crucial for managing myocardial infarction.
Purpose of the Study:
- To determine if 2-deoxyglucose (DG) accurately indicates myocardial viability during the early phases of myocardial infarction.
- To investigate the role of glucose transporters in DG uptake in infarcted rat hearts.
Main Methods:
- Autoradiography with 14C-deoxyglucose in rats with experimentally induced myocardial infarction and reperfusion.
- Assessment of infarction and risk areas using staining techniques (triphenyl tetrazolium chloride, haematoxylin-eosin, methylene blue).
- Immunohistochemical analysis for glucose transporter 1 (GLUT1) and glucose transporter 4 (GLUT4) expression.
Main Results:
- In the acute phase, 14C-DG uptake correlated with GLUT4 expression.
- In the subacute phase, DG uptake was significantly higher in both poorly viable and viable myocardium within the risk area compared to remote areas.
- GLUT1 expression was observed in poorly viable areas with inflammatory cell infiltration.
Conclusions:
- Deoxyglucose uptake can overestimate myocardial viability in the subacute phase of infarction.
- Inflammatory cell infiltration expressing GLUT1 is a key factor leading to this overestimation.
- Findings highlight the need for careful interpretation of DG uptake in assessing heart muscle viability post-infarction.
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