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Related Experiment Videos

Quaking is essential for blood vessel development.

Janice K Noveroske1, Lihua Lai, Vinciane Gaussin

  • 1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.

Genesis (New York, N.Y. : 2000)
|March 14, 2002
PubMed
Summary
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The mouse quaking gene (qk) is essential for embryonic blood vessel development, not just postnatal myelination. Defects in yolk sac vascular remodeling cause embryonic lethality.

Area of Science:

  • Developmental Biology
  • Genetics
  • Hematology

Background:

  • The mouse quaking gene (qk) has been studied for its role in postnatal central nervous system myelination.
  • Homozygous lethality of quaking alleles indicates a critical embryonic function before myelination begins.

Purpose of the Study:

  • To investigate the previously unsuspected role of the quaking gene in embryonic development.
  • To determine the cause of embryonic lethality in quaking mutants.

Main Methods:

  • Analysis of mouse embryos homozygous for the qk(k2) allele.
  • Immunohistochemical staining using antibodies against PE-CAM-1, TIE-2, SM-alpha-actin, Nkx2.5, and alpha-sarcomeric actin.
  • Assessment of embryonic heart rate in cultured embryos.

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Main Results:

  • Quaking protein is expressed in the yolk sac endoderm during early blood vessel development.
  • Mutant embryos exhibit defective yolk sac vascular remodeling and abnormal embryonic vasculature.
  • Cardiac muscle differentiation and function were normal in mutant embryos.

Conclusions:

  • The quaking gene plays an essential role in embryonic blood vessel development.
  • Defects in vascular development are the likely cause of embryonic lethality in quaking mutants.