Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The human beta-globin locus control region.

Padraic P Levings1, Jörg Bungert

  • 1Department of Biochemistry and Molecular Biology, Gene Therapy Center, Center for Mammalian Genetics, College of Medicine, University of Florida, Gainesville, FL, USA.

European Journal of Biochemistry
|March 16, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epithelial-Mesenchymal Transition Activates YAP to Drive Malignant Progression and Immune Evasion.

Cancers·2025
Same author

TFII-I/GTF2I regulates globin gene expression and stress response in erythroid cells.

The Journal of biological chemistry·2025
Same author

Toward life without sickle cell disease.

Molecular therapy : the journal of the American Society of Gene Therapy·2024
Same author

GATA2: sense and (anti)sensibility.

Blood·2024
Same author

Toxic effects of local anesthetics on rat fibroblasts: An in-vitro study.

Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association·2024
Same author

Enhancer-Mediated Formation of Nuclear Transcription Initiation Domains.

International journal of molecular sciences·2022
Same journal

Comparison of expression patterns and cell adhesion properties of the mouse biliary glycoproteins Bgp1 and Bgp2.

European journal of biochemistry·2020
Same journal

AB 3.1.1.1 (or EC 3.1.1.?).

European journal of biochemistry·2020
Same journal

Cdk5.

European journal of biochemistry·2018
Same journal

Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola.

European journal of biochemistry·2004
Same journal

Monitoring ligand-mediated nuclear receptor-coregulator interactions by noncovalent mass spectrometry.

European journal of biochemistry·2004
Same journal

Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy.

European journal of biochemistry·2004
See all related articles

The locus control region (LCR) recruits complexes to establish accessible chromatin domains for stage-specific human beta-globin gene expression. This model explains how transcription factors are loaded onto globin gene promoters.

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • The human beta-globin gene locus is crucial for oxygen transport and its expression is tightly regulated.
  • Decades of research have elucidated mechanisms of tissue- and stage-specific gene expression.
  • Integrating all known data into a comprehensive model remains challenging.

Purpose of the Study:

  • To review fundamental characteristics of globin locus regulation.
  • To present a hypothesis integrating recent findings on chromatin structure and transcription complex recruitment.
  • To explain developmental stage-specific gene expression at the beta-globin locus.

Main Methods:

  • Literature review and synthesis of existing data.
  • Formulation of a hypothesis based on recent research findings.

Related Experiment Videos

  • Conceptual division of the regulatory process into four key steps.

    • Main Results:

    • The locus control region (LCR) is proposed as a key genetic entity for efficient recruitment of regulatory complexes.
    • Recruitment of chromatin-modifying and transcription complexes establishes accessible chromatin domains.
    • Basal factors are loaded onto globin gene promoters in a developmental stage-specific manner.

    Conclusions:

    • A four-step model is proposed for beta-globin gene regulation, starting with LCR accessibility.
    • The model emphasizes the role of higher-order chromatin structure modification.
    • This mechanism facilitates developmental stage-specific transcription initiation at globin gene promoters.