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Related Experiment Videos

Cdc6 requires anchorage for its expression.

Shigeki Jinno1, Mika Yageta, Akihisa Nagata

  • 1Department of Biochemistry and Molecular Biology, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. jinno@m.u-tokyo.ac.jp

Oncogene
|March 16, 2002
PubMed
Summary
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Cell anchorage is crucial for fibroblast G1-S phase transition, regulating Cdc6 protein expression. Oncogenic factors override this requirement, enabling anchorage-independent cell cycle progression.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Oncology

Background:

  • Fibroblasts require extracellular matrix anchorage for G1 to S phase transition.
  • Oncogenic transformation alters anchorage dependence in cell cycle regulation.

Purpose of the Study:

  • To investigate the role of Cdc6 protein in anchorage-dependent cell cycle progression.
  • To elucidate the mechanisms by which oncogenic stimulation overrides anchorage requirements.

Main Methods:

  • Analysis of Cdc6 expression in rat fibroblasts upon anchorage loss and oncogenic stimulation.
  • Investigating the role of E2F-dependent promoters and cyclin-dependent kinases (CDKs).

Main Results:

  • Anchorage loss downregulates Cdc6 transcriptionally and post-transcriptionally.

Related Experiment Videos

  • Oncogenic growth factors suppress Cdc6 shutoff and activate Cdk2 and Cdk6/4.
  • Enforced Cdc6 expression bypasses anchorage requirement for G1-S transition.

    • Conclusions:

    • Cdc6 protein requires anchorage or oncogenic stimulation for its function in DNA replication origin activation.
    • G1 cyclin-dependent kinases and Cdc6 are key targets mediating anchorage loss-induced G1-S transition restriction.