An effective tool for homogeneous assays using a simple robot arm with Stackers-TRISTAN

Area of Science:

• Biotechnology
• Assay Development
• High-Throughput Screening

Background:

• Automated systems are crucial for efficient biological assays.
• Conventional robotic systems can be complex and costly for high-throughput screening.
• There is a need for user-friendly and cost-effective integrated systems for homogeneous assays.

Purpose of the Study:

• To introduce and evaluate the TANabe TRISTAN integrated system for homogeneous assays.
• To highlight the advantages of TRISTAN over conventional robotic screening systems.
• To demonstrate the effectiveness and efficiency of TRISTAN in assay development.

Main Methods:

• The TRISTAN system comprises the RapidPlate 96 (96-channel dispenser), V-MAX (plate reader), and Twister (robot arm).
• The system was developed for conducting homogeneous assays.
• TRISTAN was assessed for its performance in parameter setting, running time, hardware errors, manpower, and cost-effectiveness.

Main Results:

• The TRISTAN system demonstrated advantages in parameter setting, running time, hardware errors, manpower requirements, and cost-effectiveness compared to conventional systems.
• Despite accommodating fewer than 20 microplates, the system proved highly effective.
• The integrated nature of TRISTAN simplifies the assay workflow.

Conclusions:

• The TRISTAN integrated system is an effective and efficient solution for performing homogeneous assays.
• TRISTAN offers significant benefits for high-throughput screening, including improved cost-effectiveness and operational simplicity.
• This system represents a valuable advancement for laboratories conducting routine homogeneous assays.