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Cloning and expression of class A beta-lactamase gene blaA(BPS) in Burkholderia pseudomallei
Terence K M Cheung1, P L Ho, Patrick C Y Woo
1Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
Abstract:
The beta-lactamase gene blaA(BPS) in Burkholderia pseudomallei was cloned and expressed in Escherichia coli. BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A beta-lactamases and a relationship to the PenA (in B. cepacia) and BlaI (in Yersinia enterocolitica) lineages.
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