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Updated: Oct 9, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Published on: November 15, 2013
Fluorescence and chemical reactivity as empirical indices of matrix-induced alterations in immobilized enzymes
Abstract:
The fluorescence emission and chemical reactivity of soluble and Sepharose-bound leucine aminopeptidase and carboxypeptidase A were investigated and the results compared. Both natural fluorescence and the fluorescence of fluorescein-labeled enzymes were utilized. The front-surface viewing technique enables us to measure the apparent fluorescence quantum yields and the rotational relaxation times of fluorescent groups within native unbound and carrier-fixed enzymes. The chemical reactivity was probed with Ellman's reagent and iodoacetic acid. Both types of methods were found to be useful in detection and quantitation of matrix-induced alterations in immobilized enzymes.
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