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Related Experiment Videos

Shima drinking water--a bacteriological analysis.

P S Grover1, K Thakur

  • 1Department of Microbiology, Indira Gandhi Medical College, Shimla, Himachal Pradesh.

The Journal of Communicable Diseases
|March 20, 2002
PubMed
Summary

A year-long study found most natural water sources unsafe for consumption due to bacterial contamination. Piped water supplies, however, generally maintained good quality, indicating effective treatment and distribution.

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Area of Science:

  • Environmental microbiology
  • Public health microbiology

Background:

  • Assessing drinking water quality is crucial for public health.
  • Bacterial indicators are vital for monitoring water safety.

Purpose of the Study:

  • To bacteriologically evaluate the quality of piped water supplies and natural water sources over one year.
  • To identify the prevalence of key bacterial indicators in different water types.

Main Methods:

  • Monthly analysis of 300 water samples (60 piped, 240 natural).
  • Detection of total coliforms, Escherichia coli, and Streptococcus faecalis using the most probable number (MPN) method.
  • Isolation of Clostridium perfringens via litmus milk medium and Salmonella typhi using membrane filtration.

Main Results:

  • Piped water: Generally good quality, with only one sample unfit for consumption. Plate counts ranged from 0.5 x 10(3) to 15 x 10(3) per ml.
  • Natural sources: High bacterial loads; MPN indices for coliforms, E. coli, and S. faecalis varied significantly. Approximately one-third contained Cl. perfringens.
  • Pathogen detection: Salmonella typhi found in 1.25% of samples from natural sources. All natural sources were deemed unfit for consumption.

Conclusions:

  • Natural water sources pose a significant health risk due to widespread bacterial contamination.
  • Piped water supplies demonstrate a higher standard of safety and are generally suitable for drinking.
  • Regular monitoring of water sources is essential to ensure public health protection.

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