Effects of simulated space vacuum on bacterial cells

The effect of vacuum on bacterial cells is related to water desorption. Below water vapour pressure the inactivation remains constant, independent of total pressure and exposure time. In subsequent growth, the lag-phase of the survivors is delayed. Combined treatment with vacuum and radiation (X-rays or uv of 254 nm wavelength) results in synergistic effects, whereas vacuum and heat can act antagonistically. The vacuum inactivated cells (indicated as loss of colony-forming ability) are completely damaged. They do not show cellular elongation, phage production or respiration. The cellular membrane becomes permeable by vacuum exposure: biomolecules are released from the cells when re-suspended after vacuum treatment.