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A rapid capillary gel electrophoresis method for the quantitative determination of RuBisCo in spinach
Kumudini U K G Nicholas1, Charles F Forney, Allan T Paulson
1Agriculture and Agri-Food Canada, Atlantic Food and Horticulture Research Centre, 32 Main St., Kentville, N.S., Canada B4N IJ5.
Abstract:
A capillary gel electrophoretic (CGE) method for the quantitative analysis of RuBisCo in spinach leaves was developed. RuBisCo was resolved into large and small subunits in the presence of sodium dodecyl sulphate (SDS) by the CGE procedure which enabled the determination of the molecular weight of each unit accurately; the values so determined were in close agreement with those reported using other methods. Advantages of CGE over SDS-polyacrylamide gel electrophoresis and high-pressure gel filtration include decreased sample preparation and analysis time, superior resolution and greater sensitivity permitting reduced sample size and trace analysis. In addition, CGE provided precise quantification of RuBisCo and was demonstrated to be a viable alternative to other available methods of protein analysis.