Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative genomics by capture PCR.

Maria Lagerström-Fermér1, Dan Larhammar, Elsy Johnsen

  • 1Department of Medical Sciences, Unit of Molecular Medicine, University Hospital, Uppsala, Sweden. Maria. Lagerstrom@medsci.uu.se

Genomics
|March 20, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A ligase-based toolbox for research and diagnostics in molecular medicine.

Nucleic acids research·2026
Same author

Molecular mechanisms of native ligand selectivity in catecholamine G protein-coupled receptors.

Nature communications·2026
Same author

Evolution in metazoans of the TRPM channel family involves multiple gains and losses of genes and domains.

Molecular biology and evolution·2026
Same author

The Concise Guide to PHARMACOLOGY 2025/26: G protein-coupled receptors.

British journal of pharmacology·2025
Same author

Reformation of science publishing: the Stockholm Declaration.

Royal Society open science·2025
Same author

A denaturation-free protocol for in situ visualization of short nuclear DNA sequences using padlock probes with rolling-circle amplification.

PloS one·2025
Same journal

Integrating transcriptomics and metabolomics reveals the molecular landscape of sperm maturation driven by regional differentiation in the epididymis of Guizhou-Guiqian semi-fine wool sheep.

Genomics·2026
Same journal

Impact of genotype on histopathology and clinical characters in a Chinese cohort with obstructive hypertrophic cardiomyopathy.

Genomics·2026
Same journal

A novel reusable transcriptome-wide association study workflow used to map key genes linked to important cattle traits.

Genomics·2026
Same journal

The large mitochondrial genome of Syndiclis anlungensis (Lauraceae): Genome structure, comparative analysis, and phylogenetic relationships with other Syndiclis species.

Genomics·2026
Same journal

DeepGEP: Deep learning for gene expression prediction from multi-omics in mammals.

Genomics·2026
Same journal

Molecular features of external Auditory Canal cholesteatoma by microbial metagenomic sequencing.

Genomics·2026
See all related articles

Researchers developed a PCR method using short conserved DNA segments to find homologous genes across many species. This technique successfully isolated the somatostatin gene in vertebrates, revealing conserved sequence motifs.

Area of Science:

  • Genomics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Comparative genetic analysis aids in identifying functionally significant, evolutionarily conserved sequence features.
  • Relating genomic information across species is crucial for biological research.

Purpose of the Study:

  • To develop an efficient method for isolating homologous gene sequences from diverse species using conserved DNA segments.
  • To identify conserved sequence motifs in the 3'-untranslated region of the somatostatin gene.

Main Methods:

  • Utilized a single-sided PCR technique with short conserved coding nucleotide positions (≤32 bp).
  • Applied the method to isolate the somatostatin gene's 3'-untranslated sequence across vertebrate species.
  • Developed primers for comparative anchor tagged sequences (CATS) PCR based on conserved motifs.

Related Experiment Videos

Main Results:

  • Successfully isolated homologous somatostatin gene sequences from human to hagfish.
  • Identified two conserved sequence motifs downstream of the translational stop codon, conserved for up to 350 million years.
  • CATS PCR amplified homologous sequences from 30 out of 33 tetrapod DNA samples.

Conclusions:

  • A novel PCR procedure effectively reveals functionally relevant sequence elements.
  • The method enables primer selection for amplifying homologous sequences across a broad range of species.
  • This approach enhances cross-species genomic comparisons and gene discovery.