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Tangential migration in neocortical development
David Jiménez1, Laura M López-Mascaraque, Facundo Valverde
1Instituto Cajal (C.S.I.C.), Avenida del Doctor Arce 37, Madrid, 28002, Spain.
Developmental Biology
|March 20, 2002
Summary
Cortical development involves tangential cell migration from the basal telencephalon. This study reveals both the medial and lateral ganglionic eminences contribute to this crucial process, forming a heterogeneous cell population.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Cortical development involves tangential migration of cells from the basal telencephalon to the neocortex.
- The precise origins of these migrating cells within the ganglionic eminences remain unclear.
Purpose of the Study:
- To investigate the origins and migration routes of tangentially migrating cells during cortical development.
- To elucidate the roles of the lateral (LGE) and medial (MGE) ganglionic eminences in neocortical development.
Main Methods:
- Three-dimensional reconstruction of ganglionic eminences and interganglionic sulcus.
- In ovo and in utero cell tracing using DiI and BDA in various culture paradigms.
- Analysis of gene expression patterns (GABA, Calbindin, Calretinin) in migrating cells.
Main Results:
- Morphological development of ganglionic eminences is critical for tangential migration.
- Both LGE and MGE contribute to the neocortex, with MGE playing a confirmed role and LGE generating early migratory cells.
- Tangentially migrating cells express Calretinin and exhibit diverse spatiotemporal expression of GABA, Calbindin, and Calretinin, indicating heterogeneity.
Conclusions:
- The LGE and MGE are essential sources of tangentially migrating cells for neocortical development.
- These migrating cells constitute a heterogeneous population with distinct molecular markers and origins.