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Optimized PCR-based Detection of Mycoplasma
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An improved polymerase chain reaction assay for the detection of Tritrichomonas foetus in cattle

D Douglas Nickel1, Merle E Olson, Gilbert A Schultz

  • 1Department of Biochemistry and Molecular Biology, Health Sciences Centre, 3330 Hospital Drive NW, University of Calgary, Calgary, Alberta T2N 4N1.

The Canadian Veterinary Journal = La Revue Veterinaire Canadienne
|March 21, 2002
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...