Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Unsuspected prophage-like elements in Salmonella typhimurium.

N Figueroa-Bossi1, E Coissac, P Netter

  • 1Centre de Génétique Moleculaire, CNRS, Gif-sur-Yvette, France. Figueroa@cgm.cnrs-gif.fr

Molecular Microbiology
|July 1, 1997
PubMed
Summary

Two large excisable genetic elements, Gifsy-1 and Gifsy-2, in Salmonella typhimurium cooperate to activate recombination and excision functions. These cryptic prophages are found in multiple strains, not just LT2.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clothing and individual equipment for the female soldier: developing a framework to improve the evidence base which informs future design and evaluation.

BMJ military health·2024
Same author

Multiplexing PCR allows the identification of within-species genetic diversity in ancient eDNA.

Molecular ecology resources·2024
Same author

Foreword. History of the frontal lobe.

Revue neurologique·2018
Same author

Introduction.

Revue neurologique·2017
Same author

The evolutionary fate of the chloroplast and nuclear rps16 genes as revealed through the sequencing and comparative analyses of four novel legume chloroplast genomes from Lupinus.

DNA research : an international journal for rapid publication of reports on genes and genomes·2017
Same author

DNA metabarcoding diet analysis for species with parapatric vs sympatric distribution: a case study on subterranean rodents.

Heredity·2015

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Salmonella typhimurium harbors large excisable genetic elements.
  • These elements, Gifsy-1 and Gifsy-2, are approximately 50 kb each.
  • They are located at specific positions on the genetic map and contain rare restriction sites.

Purpose of the Study:

  • To investigate the nature and function of the Gifsy-1 and Gifsy-2 elements.
  • To understand their role in recombination and excision processes.
  • To determine their relationship with known genetic elements and their distribution in Salmonella.

Main Methods:

  • Genetic analysis of Salmonella typhimurium mutants.
  • Characterization of excisable elements Gifsy-1 and Gifsy-2.
  • Sequence similarity analysis with Escherichia coli Rac prophage recE locus.

Related Experiment Videos

  • Mutation studies to assess phenotypic effects (Sbc phenotype, sbcE mutations).
  • Prophage curing experiments to evaluate Gifsy element function.
  • Comparison with Fels prophages and analysis across different Salmonella strains.

    • Main Results:

    • Gifsy-1 and Gifsy-2 contain regions similar to the recE locus.
    • Mutations in Gifsy-1 (sbcE) suppress recB mutations by activating a recE-like gene and promoting element excision.
    • Both Gifsy-1 and Gifsy-2 are required for full activation of recombination and excision functions; curing Gifsy-2 impairs Gifsy-1 excision and suppressor activity.

    Conclusions:

    • Gifsy-1 and Gifsy-2 function as cryptic prophages.
    • They exhibit cooperative activity in regulating recombination and excision.
    • These elements are not strain-specific to S. typhimurium LT2 and are present in other virulent isolates.