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Blocking transforming growth factor-beta receptor signaling down-regulates transforming growth factor-beta1
Wei Liu1, Zehao Cai, Danru Wang
1Department of Plastic and Reconstructive Surgery, Shanghai Institute of Plastic and Reconstructive Surgery, Tissue Engineering Center, Shanghai 9th People's Hospital, Shanghai Second Medical University, Shanghai 200011, China. liuwei-2000@yahoo.com
Objective:
To study transforming growth factor-beta1 (TGF-beta1) autoproduction in keloid fibroblasts and the regulation effect of blocking TGF-beta intracellular signaling on rhTGF-beta1 autoproduction.
Methods:
Keloid fibroblasts cultured in vitro were treated with either rhTGF-beta1 (5 ng/ml) or recombinant adenovirus containing a truncated type II TGF-beta receptor gene (50 pfu/cell). Their effects of regulating gene expression of TGF-beta1 and its receptor I and II were observed with Northern blot.
Results:
rhTGF-beta1 up-regulated the gene expression of TGF-beta1 and receptor I, but not receptor II. Over-expression of the truncated receptor II down-regulated the gene expression of TGF-beta1 and its receptor I, but not receptor II.
Conclusions:
TGF-beta1 autoproduction was observed in keloid fibroblasts. Over-expression of the truncated TGFbeta receptor II decreased TGF-beta1 autoproduction via blocking TGF-beta receptor signaling.