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High resolution comparative genomic hybridization detects 7-8 megabasepair deletion in PCR amplified DNA
J Larsen1, A M Ottesen, M Kirchhoff
1Finsen Laboratory, Finsen Center, Rigshospitalet, Strandboulevarden 49, DK-2100, Copenhagen, Denmark. jacob.larsen@finsenlab.dk
Abstract:
We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP-PCR) as opposed to the use of unamplified DNA. Five DNA samples from B-cell leukemias with small 11q deletions were amplified by DOP-PCR and analysed by means of high resolution comparative genomic hybridization (HR-CGH) for the evaluation of aberration size detection limit. By means of HR-CGH, we found the detection limit of DOP-PCR CGH for deletions to be between 3 Mbp and 7-8 Mbp.