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The use of dubious cell lines in research: is trust enough?

C F Arlett

    The Lancet. Oncology
    |March 22, 2002
    PubMed
    Abstract

    No abstract available in PubMed .

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    A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...