Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recombinant CD40 ligand administration does not decrease intensity of Pneumocystis carinii infection in scid mice.

V H Vestereng1, J A Kovacs

  • 1Critical Care Medicine Department, National Institutes of Health, Bethesda, MD, USA.

The Journal of Eukaryotic Microbiology
|March 22, 2002
PubMed
Summary

X-linked Hyper IgM Syndrome (HIM) is a rare immunodeficiency linked to CD40 ligand mutations. Treatment with CD40 ligand alone did not clear Pneumocystis carinii pneumonia in mice, indicating its insufficiency for infection resolution.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cycling of gut mucosal CD4+ T cells decreases after prolonged anti-retroviral therapy and is associated with plasma LPS levels.

Mucosal immunology·2009
Same author

Pneumocystis carinii: a fungus resistant to antifungal therapies - mechanisms of action of antipneumocystis drugs.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2006
Same author

A comparison of microLC/electrospray ionization-MS and GC/MS for the measurement of stable isotope enrichment from a [2H2]-glucose metabolic probe in T-cell genomic DNA.

Analytical chemistry·2003
Same author

New insights into transmission, diagnosis, and drug treatment of Pneumocystis carinii pneumonia.

JAMA·2001
Same author

Identification of dynamically distinct subpopulations of T lymphocytes that are differentially affected by HIV.

The Journal of experimental medicine·2001
Same author

Characterization of the expression site of the major surface glycoprotein of human-derived Pneumocystis carinii.

Molecular microbiology·2001

Area of Science:

  • Immunology
  • Infectious Diseases
  • Genetics

Background:

  • X-linked Hyper IgM Syndrome (HIM) is a primary immunodeficiency caused by CD40 ligand gene mutations.
  • Patients with HIM exhibit susceptibility to opportunistic infections like Pneumocystis carinii pneumonia.
  • CD40 ligand (CD40L) plays a crucial role in immune responses and host defense.

Purpose of the Study:

  • To investigate the efficacy of murine recombinant CD40 ligand trimer (muCD40L) in clearing Pneumocystis carinii infection.
  • To evaluate CD40L as a potential therapeutic agent against P. carinii pneumonia in an animal model.

Main Methods:

  • Severe combined immunodeficient (SCID) mice were infected with Pneumocystis carinii.
  • Treated group received daily administration of muCD40L for 21 days.

Related Experiment Videos

  • Organism burden was assessed and compared between treated and control groups.

    • Main Results:

    • No significant difference in Pneumocystis carinii organism burden was observed between muCD40L treated mice and control mice.
    • Treatment with muCD40L alone did not lead to the clearance of P. carinii infection in this experimental model.

    Conclusions:

    • Murine recombinant CD40 ligand trimer monotherapy is ineffective in clearing Pneumocystis carinii infection in SCID mice.
    • CD40L alone is insufficient for resolving P. carinii pneumonia, suggesting the need for combination therapies or alternative strategies.