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Optimization of some additives to improve protease production under SSF
R Tunga1, R Banerjee, B C Bhattacharyya
1Department of Agricultural & Food Engineering, Indian Institute of Technology, Kharagpur.
Indian Journal of Experimental Biology
|March 22, 2002
Summary
This study found that Tween-80 and dioctyl sodium sulfosuccinate significantly enhanced protease production in Rhizopus oryzae. Biotin, Ca2+, and 1-Naphthyl acetic acid also boosted enzyme synthesis during solid-state fermentation.
Area of Science:
- Microbiology
- Enzymology
- Biotechnology
Background:
- Proteases are essential enzymes with diverse industrial applications.
- Optimizing protease production is crucial for cost-effective industrial use.
- Rhizopus oryzae is a known protease-producing microorganism.
Purpose of the Study:
- To identify optimal conditions for enhancing protease production by Rhizopus oryzae.
- To investigate the effect of specific additives on protease synthesis via solid-state fermentation.
Main Methods:
- Solid-state fermentation (SSF) using a locally isolated Rhizopus oryzae strain.
- Cultivation on wheat bran as a substrate.
- Testing the impact of various concentrations of Tween-80, dioctyl sodium sulfosuccinate, biotin, Ca2+, and 1-Naphthyl acetic acid.
Main Results:
- Highest protease yield (388.54 U/g wheat bran) was achieved with Tween-80 and dioctyl sodium sulfosuccinate at 40 mg/g wheat bran.
- Biotin (0.0025 mg/g), Ca2+ (0.05 mg/g), and 1-Naphthyl acetic acid (0.01 mg/g) demonstrated an inducing effect on protease synthesis.
Conclusions:
- Specific surfactants and micronutrients can significantly enhance protease production in Rhizopus oryzae.
- Solid-state fermentation parameters can be optimized using cost-effective additives for improved enzyme yield.