Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Staphylococcal enterotoxin C: solid-phase radioimmunoassay.

J A Bukovic, H M Johnson

    Applied Microbiology
    |October 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The chromosomal locations of leaf peroxidase genes in hexaploid wheat, rye and barley.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same author

    Dermatitis herpetiformis.

    Archives of dermatology and syphilology·2010
    Same author

    Nevus unius lateris.

    Archives of dermatology and syphilology·2010
    Same author

    Lichen striatus.

    Archives of dermatology and syphilology·2010
    Same author

    Sporotrichosis.

    Archives of dermatology and syphilology·2010
    Same author

    Lichen nitidus.

    Archives of dermatology and syphilology·2010
    Same journal

    Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents.

    Applied microbiology·1975
    Same journal

    Microbial indicators of oil-rich salt marsh sediments.

    Applied microbiology·1975
    Same journal

    Accelerated Fermentation of Brewer's Wort by Saccharomyces carlsbergensis.

    Applied microbiology·1975
    Same journal

    Semisolid fermentation of ryegrass straw.

    Applied microbiology·1975
    Same journal

    Microbiology of ripening honey.

    Applied microbiology·1975
    Same journal

    Microwave sanitization of color additives used in cosmetics: feasibility study.

    Applied microbiology·1975
    See all related articles

    A new radioimmunoassay accurately detects staphylococcal enterotoxin C in various foods. This method is sensitive and reliable for ensuring food safety.

    Area of Science:

    • Food safety and analytical chemistry.
    • Microbiology and immunology.

    Background:

    • Staphylococcal enterotoxins are a major cause of foodborne illness.
    • Accurate detection of enterotoxins in diverse food matrices is crucial for public health.

    Purpose of the Study:

    • To develop and validate a sensitive assay for detecting staphylococcal enterotoxin C.
    • To quantitate enterotoxin C levels in common food products.

    Main Methods:

    • A solid-phase radioimmunoassay (RIA) was developed.
    • The assay utilized 125I-labeled enterotoxin C and antibody-coated polystyrene tubes.
    • The method was applied to condensed milk, cheddar cheese, custard, and ham salad.

    Main Results:

    • The radioimmunoassay demonstrated high sensitivity, detecting 1 to 10 ng of enterotoxin C per gram of food.

    Related Experiment Videos

  • Nonspecific inhibitions were minimal, recorded at 16% or less, indicating assay specificity.
  • The assay successfully quantified enterotoxin C in tested food samples.

    • Conclusions:

    • The developed solid-phase radioimmunoassay is a viable method for detecting and quantifying staphylococcal enterotoxin C in various foods.
    • This assay provides a sensitive and specific tool for food safety monitoring and quality control.
    • The findings contribute to preventing foodborne illnesses associated with enterotoxin C contamination.