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Morphological and physiological changes in Streptomyces lividans induced by different yeasts
Ramón I Santamaría1, Fernando Leal, Margarita Díaz
1Instituto de Microbiología Bioquímica, Consejo Superior de Investigaciones Científicas (CSIC)/Universidad de Salamanca, Edificio Departamental, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain. santa@usal.es
Archives of Microbiology
|March 22, 2002
Summary
Interactions between Streptomyces and yeasts alter Streptomyces development. Yeast-produced invertase breaks down sucrose, providing glucose and fructose for Streptomyces growth, impacting spore formation.
Area of Science:
- Microbiology
- Developmental Biology
- Biochemistry
Background:
- Streptomyces development culminates in the formation of unigenomic spores from aerial hyphae.
- Intraspecific and interspecific microbial interactions are crucial for regulating Streptomyces developmental pathways.
Purpose of the Study:
- To investigate the impact of yeast-microorganism interactions on Streptomyces developmental programs.
- To elucidate the biochemical mechanisms underlying observed developmental alterations.
Main Methods:
- Co-cultivation of Streptomyces with yeast species.
- Analysis of Streptomyces morphological development.
- Enzymatic assays to detect invertase activity and substrate utilization.
Main Results:
- Co-cultivation with yeast significantly altered Streptomyces developmental trajectories.
- Yeast invertase activity was confirmed, leading to the hydrolysis of sucrose.
- Increased availability of glucose and fructose in the culture medium was observed, supporting enhanced Streptomyces growth.
Conclusions:
- Yeast-derived invertase plays a critical role in modulating Streptomyces development.
- The availability of simple sugars, facilitated by yeast, influences the Streptomyces life cycle and spore formation.
- Microbial interactions can reprogram complex developmental processes in bacteria.