Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane capacitance measurement using patch clamp with integrated self-balancing lock-in amplifier.

Stuart L Johnson1, Martin V Thomas, Corné J Kros

  • 1School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.

Pflugers Archiv : European Journal of Physiology
|March 22, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Increased head-turning, hyperactivity and low-penetrance circling behaviour in mice lacking ZPLD1, a protein that scaffolds the cupula to the roof of the ampulla.

Hearing research·2026
Same author

Myosin 7a is required for maintaining the transducing stereocilia and for force transmission to the MET channel during cochlear hair cell development.

The Journal of physiology·2026
Same author

Adeno-associated virus-based rescue of Myo7a expression restores hair-cell function and improves hearing thresholds in a USH1B mouse strain.

The Journal of physiology·2025
Same author

Mechanisms driving the functional maturation of the developing mammalian auditory pathway.

Current topics in developmental biology·2025
Same author

Transient capacitance changes recorded from vestibular type I hair cells are produced by G<sub>K,L</sub> gating and do not involve neurotransmitter exocytosis.

The Journal of physiology·2025
Same author

MYO7A is required for the functional integrity of the mechanoelectrical transduction complex in hair cells of the adult cochlea.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Expression of protein kinase A catalytic subunits in healthy and diseased mouse kidneys.

Pflugers Archiv : European journal of physiology·2026
Same journal

Localization of O₂‑sensing ADO‑RGS pathway components in mouse and human kidneys under normoxia, hypoxia and renal fibrosis.

Pflugers Archiv : European journal of physiology·2026
Same journal

Cholesterol-depleting dextrin depolarizes sarcolemma: the contribution of sodium / calcium channels and membrane integrity.

Pflugers Archiv : European journal of physiology·2026
Same journal

Inhibition of (interstitial) P2Y<sub>6</sub> receptors attenuates fibrosis progression.

Pflugers Archiv : European journal of physiology·2026
Same journal

Renal NHE3 is required to limit hypokalemia and metabolic acidosis during dietary potassium deficiency.

Pflugers Archiv : European journal of physiology·2026
Same journal

Xenin-25 improves indomethacin-induced acute gastric injury in rats.

Pflugers Archiv : European journal of physiology·2026
See all related articles

A novel "track-in" mode integrates lock-in amplifiers with patch clamp circuitry for precise exocytosis monitoring. This method enhances cell membrane capacitance measurements, offering improved accuracy and convenience.

Area of Science:

  • Biophysics
  • Cell Biology
  • Neuroscience

Background:

  • Patch clamp techniques are crucial for studying cell membrane properties.
  • Monitoring exocytosis requires sensitive measurement of cell membrane capacitance.
  • Conventional methods can be sensitive to phase errors and complex to implement.

Purpose of the Study:

  • To develop a novel operating mode for patch clamp setups.
  • To improve the accuracy and convenience of exocytosis monitoring.
  • To integrate lock-in amplifier technology with resistance and capacitance compensation circuitry.

Main Methods:

  • Incorporated a lock-in amplifier into patch clamp resistance and capacitance compensation circuits.
  • Developed the
  • track-in

Related Experiment Videos

  • mode using lock-in amplifier outputs for electronic adjustments.
  • Utilized model cells and mouse inner hair cells for validation.

    • Main Results:

    • Achieved linear and calibrated resistance and capacitance measurements.
    • Maintained sensitivity and time resolution comparable to conventional techniques.
    • Effectively eliminated sensitivity to switching phase errors.
    • Demonstrated advantages over software implementations in economy and convenience.

    Conclusions:

    • The
    • track-in
    • mode offers a robust and user-friendly approach for exocytosis studies.
    • Direct recording of capacitance signals simplifies data acquisition.
    • This technique enhances the utility of patch clamp electrophysiology for cellular research.