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Related Concept Videos

Transcription01:10

Transcription

159.5K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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Transcription01:10

Transcription

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No description available
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Transcription01:17

Transcription

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Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
36.3K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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Transcription01:17

Transcription

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No description available
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Related Experiment Video

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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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Integrating mRNA processing with transcription.

Nick J Proudfoot1, Andre Furger, Michael J Dye

  • 1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom. nicholas.proudfoot@path.ox.ac.uk

Cell
|March 23, 2002
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Summary
This summary is machine-generated.

Messenger RNA (mRNA) processing events like capping, splicing, and polyadenylation happen during transcription. These processes are coordinated by transcription and influence each other

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Area of Science:

  • Molecular Biology
  • Gene Expression
  • RNA Processing

Background:

  • Messenger RNA (mRNA) processing, including capping, splicing, and polyadenylation, are crucial steps in gene expression.
  • These processing events are known to occur during the transcription of genes.
  • The coordination and interplay between these reactions are not fully understood.

Purpose of the Study:

  • To elucidate the cotranscriptional nature of mRNA processing.
  • To investigate the coordination between capping, splicing, and polyadenylation.
  • To understand the role of RNA polymerase II in facilitating these integrated processes.

Main Methods:

  • Analysis of cotranscriptional mRNA processing pathways.
  • Investigating the molecular interactions between transcription and mRNA processing factors.
  • Studying the function of the phosphorylated C-terminal domain (CTD) of RNA polymerase II.

Main Results:

  • mRNA capping, splicing, and polyadenylation occur concurrently with transcription.
  • These processing reactions exhibit mutual influence on their efficiency and specificity.
  • Transcription actively coordinates these mRNA processing events.
  • The phosphorylated CTD of RNA polymerase II serves as a platform for key molecular interactions with processing factors.

Conclusions:

  • mRNA processing is an integrated cotranscriptional process.
  • Transcription plays a central role in coordinating mRNA capping, splicing, and polyadenylation.
  • RNA polymerase II's CTD is essential for linking transcription with mRNA maturation.