[Studies on bioconversion conditions and stereoselectivity of Arthrobacter K1108 hydantoinase]

The bioconversion conditions of hydantoinase of Arthrobacter K1108 were studied. It was shown that the optimum temperature of the enzyme is 55 degrees C, and the optimum pH is 7.0. The enzyme can be activated by Co2+ and Fe2+, while inhibited by Ca2+. The optimal substrate of the hydantoinase is 5-benzylhydantoin, while 5-phenylhydantoin and 5-indolylmethylhydantoin cannot effectively digested, showing a high specificity on the substrates. An investigation on the hydantoinase stereoselectivity mechanism showed that the N-carbamoylamino acid hydrolase is stereoselective but the hydantoin hydrolase is not.