Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD105 (endoglin) expression on hematopoietic stem/progenitor cells.

L Pierelli1, G Bonanno, S Rutella

  • 1Istituto di Ostetricia e Ginecologia, Universita' Cattolica S. Cuore, Roma, Italy. luca@pierelli.it

Leukemia & Lymphoma
|March 26, 2002
PubMed
Summary

Endoglin (CD105) marks primitive hematopoietic stem cells. Transforming growth factor-beta 1 (TGF-beta1) maintains CD105 expression, influencing cell immaturity and differentiation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

7 Tesla MRI in Multiple Sclerosis: Insights From Its Use in Clinical Routine.

European journal of neurology·2025
Same author

Muographic monitoring of the volcano-tectonic evolution of Mount Etna.

Scientific reports·2020
Same author

Potency testing of mesenchymal stromal cell growth expanded in human platelet lysate from different human tissues.

Stem cell research & therapy·2016
Same author

Culture of human cell lines by a pathogen-inactivated human platelet lysate.

Cytotechnology·2015
Same author

Role of calpain-1 in the early phase of experimental ALS.

Archives of biochemistry and biophysics·2014
Same author

Acute stress rapidly increases the readily releasable pool of glutamate vesicles in prefrontal and frontal cortex through non-genomic action of corticosterone.

Molecular psychiatry·2014

Area of Science:

  • Hematology
  • Cell Biology
  • Molecular Biology

Background:

  • Endoglin (CD105) is a transforming growth factor-beta receptor (TGF-betaR) accessory molecule, with poorly understood roles in receptor-ligand interactions.
  • A subset of CD34+ hematopoietic stem/progenitor cells expresses CD105, exhibiting features of primitive hematopoietic cells.
  • These CD34+/CD105+ cells are found in peripheral blood and can be isolated using immunomagnetic strategies.

Purpose of the Study:

  • To investigate the role of endoglin (CD105) in primitive hematopoiesis.
  • To understand the influence of transforming growth factor-beta 1 (TGF-beta1) on CD105 expression and hematopoietic stem cell characteristics.

Main Methods:

  • Flow cytometry to analyze cell surface markers (CD105, CD34, lineage markers).

Related Experiment Videos

  • Immunomagnetic isolation of specific cell populations (CD34+/CD105+ and CD34-Lin-).
  • In vitro culture of hematopoietic cells with cytokines (Flt3 ligand, erythropoietin, interleukin-15, vascular endothelial growth factor) and TGF-beta1.

    • Main Results:

    • CD34+/CD105+ cells possess hematopoietic potential sustained by various cytokines.
    • Endogenous TGF-beta1 is critical for maintaining CD34+/CD105+ cell immaturity by upregulating CD105 and CD34 expression.
    • TGF-beta1 exposure induces CD105 expression on primitive CD34-Lin- cells, suggesting a role in their differentiation.
    • CD105 expression appears to modulate TGF-beta1's effects on cell cycle, survival, and differentiation.

    Conclusions:

    • Endoglin (CD105) expression is a marker for primitive hematopoietic stem cells.
    • TGF-beta1 plays a key role in regulating CD105 expression and maintaining hematopoietic stem cell immaturity.
    • CD105 may mediate distinct TGF-beta1 signaling pathways affecting cell cycle, survival, and differentiation in hematopoiesis.