Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-speed plasmid isolation using 96-well, size-exclusion filter plates.

D Harris1, M Engelstein, R Parry

  • 1Millipore, Danvers, MA 01923, USA. dylan_harris@millipore.com

Biotechniques
|March 26, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolutionary principles underlying neuron subtype encoding and diversification in animals.

bioRxiv : the preprint server for biology·2026
Same author

Prediction-based attention computing: a proof of concept study.

Virtual reality·2026
Same author

Exploring the association between suicidal thoughts, self-injury, and GLP-1 receptor agonists in weight loss treatments: Insights from pharmacovigilance measures and unmasking analysis.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology·2024
Same author

Qualitative studies conducted alongside randomized controlled trials in oncology: A scoping review of use and rigour of reporting.

International journal of nursing studies·2022
Same author

Adult North Star Network (ANSN): Consensus Document for Therapists Working with Adults with Duchenne Muscular Dystrophy (DMD) - Therapy Guidelines.

Journal of neuromuscular diseases·2022
Same author

Putative intranuclear coccidium in Mauremys leprosa (Schweigger) from Morocco.

Folia parasitologica·2021

This study presents a rapid, 30-minute method for isolating plasmid DNA using size-exclusion filtration. This fast DNA purification technique is ideal for high-throughput genomics applications like DNA sequencing.

Area of Science:

  • Molecular Biology
  • Genomics

Background:

  • Genomic analysis requires rapid DNA sample preparation.
  • Traditional plasmid DNA isolation methods are time-consuming.

Purpose of the Study:

  • To develop a rapid, size-exclusion-based system for plasmid DNA isolation.
  • To optimize plasmid DNA purification for high-throughput applications.

Main Methods:

  • Developed a 96-well microplate system for plasmid DNA isolation.
  • Utilized modified alkaline lysis for bacterial lysate preparation.
  • Employed vacuum filtration and size-exclusion chromatography.

Main Results:

  • Achieved plasmid DNA isolation in 30 minutes.
  • Eliminated the need for chaotropic salts and ethanol.

Related Experiment Videos

  • Demonstrated high yields, reproducibility, and quality of purified plasmids.

    • Conclusions:

    • The developed system offers a fast and efficient method for plasmid DNA purification.
    • This protocol is suitable for cloning and DNA sequencing operations.
    • The size-exclusion system accelerates genomics analysis workflows.