Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cytoplasm

Cytoplasm

The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.Protein Folding and MisfoldingThe cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein core...

Introduction to the Cytoskeleton

Introduction to the Cytoskeleton

Overview of the Cytoskeleton
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ̶ microfilaments, microtubules, and intermediate filaments. Each has characteristic features that distinguish them, including the dynamics of their assembly and disassembly, mechanical properties, polarity, and the type of molecular motors associated with them. Earlier, they were thought to be present only in eukaryotic cells; however, their homologs were...

Adaptability of Cytoskeletal Filaments

Adaptability of Cytoskeletal Filaments

The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...

Polarity of the Cytoskeleton

Polarity of the Cytoskeleton

The intrinsic polarity of cells can be primarily attributed to two factors- i) the asymmetric accumulation of mobile components such are regulatory molecules and subcellular components across the cell and ii) the orientation of polar cytoskeletal filaments that make up the cytoskeletal networks, specifically microfilaments, and microtubules arranged along the axis of polarity. Interactions between the cytoskeletal filaments are crucial for the establishment and maintenance of the polar nature...

Cytoskeletal Coordination in Cell Migration

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...

Cytoplasm

Cytoplasm

The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.
Protein Folding and Misfolding
The cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

T Cell Dysfunction in the Acidic Tumor Microenvironment.

Acta physiologica (Oxford, England)·2026

Same author

Epithelium dynamics differ in time and space when exposed to the permeation enhancers penetramax and EGTA. A head-to-head mechanistic comparison.

Frontiers in drug delivery·2025

Same author

The SLC9A-C Mammalian Na<sup>+</sup>/H<sup>+</sup> Exchanger Family: Molecules, Mechanisms, and Physiology.

Physiological reviews·2019

Same author

Roles of pH in control of cell proliferation.

Acta physiologica (Oxford, England)·2018

Same author

Tumor microenvironment conditions alter Akt and Na<sup>+</sup>/H<sup>+</sup> exchanger NHE1 expression in endothelial cells more than hypoxia alone: implications for endothelial cell function in cancer.

BMC cancer·2017

Same author

Disrupting Na⁺, HCO₃⁻-cotransporter NBCn1 (Slc4a7) delays murine breast cancer development.

Oncogene·2015

Search research articles

Related Experiment Video

Updated: Jul 11, 2026

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Published on: December 3, 2010

The cytoskeleton and cell volume regulation.

S F Pedersen¹, E K Hoffmann, J W Mills

¹Biochemistry Department, August Krogh Institute, Copenhagen, Denmark. sfpedersen@aki.ku.dk

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology

|March 27, 2002

Summary

Cell volume regulation involves complex signaling pathways. In Ehrlich ascites tumor cells, F-actin content changes with cell volume, but cytochalasin B

More Related Videos

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

Published on: August 27, 2015

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Published on: March 10, 2023

Related Experiment Videos

Last Updated: Jul 11, 2026

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Published on: December 3, 2010

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

Published on: August 27, 2015

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Published on: March 10, 2023

Area of Science:

Cell Biology
Biochemistry
Physiology

Background:

Osmotic signal transduction involves receptor clustering and downstream signaling.
The F-actin cytoskeleton plays a role in cell volume regulation.
Ehrlich ascites tumor cells (EATC) are a model for studying cell volume regulation.

Purpose of the Study:

To investigate the relationship between signaling events, F-actin cytoskeleton, and volume-regulatory transporters.
To examine the effects of cytochalasins on F-actin content and cell volume regulation in EATC.

Main Methods:

Review of recent evidence on signaling events and F-actin.
Experimental work in Ehrlich ascites tumor cells (EATC).
Treatment with cytochalasins (cytochalasin B and D) to disrupt F-actin.

Main Results:

EATC cell shrinkage increases F-actin content; cell swelling decreases it.
Cytochalasin treatment effects on F-actin content do not always correlate with F-actin organization or cell morphology.
Cytochalasin B, but not cytochalasin D, inhibited regulatory volume decrease (RVD) and regulatory volume increase (RVI) at concentrations that depolymerize F-actin.

Conclusions:

The effect of cytochalasin B on RVD and RVI in EATC may involve mechanisms beyond simple F-actin depolymerization, potentially F-actin severing.
Further research is needed to elucidate the precise mechanisms of osmotic signal transduction and F-actin's role.