Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cl(-)-ATPases: biological active transporters.

G A Gerencser1, J Zhang

  • 1Department of Physiology and Department of Medicine, College of Medicine, University of Florida, Gainesville 32610-0274, USA.

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
|March 27, 2002
PubMed
Summary

This study investigates chloride transport mechanisms, finding evidence that chloride-stimulated ATPases may actively transport chloride across plasma membranes, a process needing further molecular confirmation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sodium-Sulfate Symport by Aplysia californica Gut.

Zoological science·2008
Same author

Transport energetics of the Na+ pump in Aplysia californica gut.

Canadian journal of physiology and pharmacology·2001
Same author

Effect of Laticauda semifasciata (sea snake) venom on chloride transport across the frog skin.

Toxicon : official journal of the International Society on Toxinology·2001
Same author

Phosphorylation of chloride-ATPase reconstituted from Aplysia gut.

The Journal of experimental zoology·2001
Same author

The Aplysia californica Cl- pump is a P-type ATPase: evidence through inhibition studies.

Canadian journal of physiology and pharmacology·2001
Same author

Intracellular K+ activities in Aplysia gut: effects of transport inhibitors on the Na+ pump.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2001

Area of Science:

  • Biochemistry
  • Cell Biology
  • Membrane Transport

Background:

  • Five established mechanisms for plasma membrane chloride transport exist.
  • Primary active chloride transport lacks genetic evidence, despite co-existing cellular Cl(-)-stimulated ATPases and uphill chloride transport.
  • Cl(-)-stimulated ATPase activity is widespread in cells, notably in mitochondria and plasma membranes.

Purpose of the Study:

  • To explore the potential role of Cl(-)-stimulated ATPases in primary active chloride transport.
  • To investigate the mediation of net chloride movement against its electrochemical gradient by ATPases.
  • To address the gap in genetic evidence for active chloride transport mechanisms.

Main Methods:

  • Analysis of Cl(-)-stimulated ATPase activity in cellular systems and liposomes.

Related Experiment Videos

  • Examination of uphill chloride transport in conjunction with ATPase activity.
  • Review of existing literature on chloride transport and ATPase functions.

    • Main Results:

    • Cellular Cl(-)-stimulated ATPases are frequently found alongside unexplained uphill chloride transport.
    • Plasma membranes exhibit Cl(-)-stimulated ATPase activity.
    • Studies suggest ATPases may mediate net chloride movement up its electrochemical gradient across plasma membranes.

    Conclusions:

    • Cl(-)-stimulated ATPases show potential as mediators of primary active chloride transport at the plasma membrane.
    • Further molecular biological studies are essential to confirm the direct transport role of plasma membrane-localized Cl(-)-stimulated ATPases.
    • The findings challenge existing models by proposing a novel mechanism for active chloride transport.