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Molecular cloning of cDNA encoding polypeptide chain elongation factor 1alpha from goldfish (Carassius auratus)

M Tokumoto1, Y Nagahama, T Tokumoto

  • 1Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan. sbttoku@ipc.shizuoka.ac.jp

DNA Sequence : the Journal of DNA Sequencing and Mapping
|March 27, 2002
PubMed

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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